The virtual gel electrophoresis in SeqBuilder Pro is amazing. Image Source: DNASTAR Gel Electrophoresis Simulation The software provides custom vector catalogs that provide annotated plasmid vector maps for in silico cloning. There is an in-built tool for automatic annotation of plasmid using database features. Image Source: DNASTAR Plasmid MapsĪn interesting feature of SeqBuilder Pro is to offer an easy interface to create and annotate graphically rich maps for cloning and publication. The workflow of verification catches the read misalignments and errors in insertions before moving towards the downstream experiments.

The identified discrepancies are immediately addressed by it. The clone verification protocol by SeqBuilder Pro checks the correct orientation of the insert, adequate coverage of trace files, etc. Image Source: DNASTAR Clone Sequence VerificationĪfter you have created a clone in your lab, you must validate your results whether it was done appropriately or not. The step-by-step guide helps in preparing the vectors and amplifying the inserts by different cloning methods ( Gateway, multisite, GeneArt, TA, and TOPO). The ability to batch clone groups of fragments simultaneously is possible here. SeqBuilder Pro offers the flexibility to meet the experiment requirements. Image Source: DNASTAR Automatic Virtual Cloningīefore trying your hands at the lab with clone designing, you need to know what the clone would be like. Even the first time users can rely on this for saving time and resources. The choice of editing primer through customization, editing, and mutating is beneficial. SeqBuilder Pro provides this unique ability to design a virtual primer before performing the PCR in wet labs. However, any error in the initial step of Primer designing can ruin the entire setup. Image Source: DNASTAR Primer Design for PCRĪ perfectly designed primer is the key to setting up successful PCR experiments. The editing can be done fast due to this.

The process is automatic and the steps are simple. It provides the ability to change the nucleotides, amino acids, ORF identification, and addition of restriction sites. You can utilize this comprehensive editing software for change in sequences. Sequence editing and annotation is a primary step for molecular biology research. Some key features or programs executed by the software include: Sequence Editing and AnnotationĪ powerful feature of the SeqBuilder Pro for sequence analysis. The SeqBuilder Pro package is a small part of the Lasergene Molecular Biology package, but it comprises the basic sequence analysis tools. Let’s talk about the key features of the software in the upcoming section. However, a little deeper insight into the software’s abilities would let us know if it is worth the price.

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